At the end of this assignment, you should be able to:
- Explain how the m/z values and spacing of peaks in an ESI charge envelope is used to determine molecular weight.
- Calculate the charge state and mass of a protein from its ESI-MS spectrum.
- Explain how a quadrupole ion trap mass analyzer works.
Calculating the molecular weight of a protein from its electrospray ionization mass spectrum (ESI-MS)
References and Resources
- G.L. Glish and R.W. Vachet, “The basics of mass spectrometry in the twenty-first century,” Nat. Rev. Drug Discov., 2003, 2, 140-150.
- C. Hendrickson, “Electrospray Ionization (ESI)”, https://nationalmaglab.org/user-facilities/icr/techniques/esi
- C.S. Ho, et al. “Electrospray ionization mass spectrometry: principles and clinical applications,” Clin. Biochem. Rev. 2003, 24, 3-12.
- J.R. Yates, “A century of mass spectrometry: from atoms to proteomes,” Nat. Methods, 2011, 8, 633-637.
- J. Yergey, et al. “Isotopic distributions in mass spectra of large molecules,” Anal. Chem. 1983, 55, 353-358.