Appendix 1
- Page ID
- 222850
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)Guidelines for Using the Cary 100 UV-Visible Spectrophotometer to Obtain Beer's Law and Kinetics Data
To launch the software click on the icon Cary WinUV. Follow the column on the left of the table below for common commands and the details for the particular experiment in the appropriate column to the right. Note: items not checked off on the screen or not highlighted for change are not listed below.
| Day 1 | Day 2 | Day 3 | |
| Experiment | Beer's Law | Scanning Kinetics | Kinetics @550 nm |
| Click on the icon: | Scan | Scanning Kinetics | Kinetics |
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On the menu bar click on File then on Open Method select: |
5_311_Lambert- Beer_Cobalt Click on Open |
5_311Scanning KineticsCobalt Click on Open |
5_311KineticsCobalt Click on Open |
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Click on the Setup button. Check that the parameters are correct. See below: |
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Setup: Cary |
X mode:nanometers Start: 700.00 nm Stop: 300.00 nm Y min: 0.00 Ymax: 1.20 Scan Controls Temperature Monitor: Block x Show Status Display |
X mode:nanometers Start: 700.00 nm Stop: 300.00 nm Y min: 0.00 Ymax: 1.00 Scan Controls Collect Timing: x Show Status Display |
Wavelength(nm): 550.00 Collect Timing: x Show Status Display |
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Set-up: Options |
SBW(nm): 2.0 Source: UV-VIS Source changeover: 350 nm x Show Status Display |
x Auto Lamp Off SBW(nm): 2.0 Energy: 1.00 Source: UV-VIS Source changeover: 350 nm x Show Status Display |
x Auto Lamp Off Source: VIS Display |
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Setup: Baseline |
> Baseline correction x Show Status Display |
> Baseline correction x Show Status Display |
Not available |
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Setup: Accesories 1 |
Cells: Temperature Setting Controller Block 25.0°C Temperature Display: x Block Display |
Cells: Temperature Setting Controller Block 60.0°C Temperature Display: x Block Display |
Cells: Temperature Setting Controller Display |
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Setup: Accessories 2 |
Not Used | Not Used | Not Used |
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Setup: Accessories 3 |
Not Used | Not Used | Not Used |
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Setup: Analyze |
Not Available | Not Used | Not Used |
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Setup: Accessories 3 |
Not Available | Not Available |
Fill in: |
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Setup: Reports |
Operator: Options: > All traces Peaks XY Pairs Table: Table Interval Autoconvert: > Select for ASCII(csv) x Show Status Display |
Operator: Options: XY Pairs Table: Table Autoconvert: > Select for ASCII(csv) x Show Status Display |
Operator: Options: XY Pairs Table: Table Autoconvert: > Select for ASCII(csv) x Show Status Display |
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Setup: Autostore |
> Storage on (prompt at start) x Show Status Display |
> Storage on (prompt at start) x Show Status Display |
> Storage on (prompt at start) x Show Status Display |
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Setup: Click on the OK button. Wait for the green traffic light. |
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To obtain the baseline click on Baseline button Name cell 1 "0.5M HNO3" Wait while the graph of Absorbance vs wavelength appears on the screen. Wait for the green traffic light. |
To obtain the baseline click on Baseline button Name cell 1 "0.5M HNO3" Wait while the graph of Absorbance vs wavelength appears on the screen. Wait for the green traffic light. |
Baseline correction is not used. To zero the instrument at 550 nm click on Zero then: Name cell 1 "0.5M HNO3" Insert cuvet filled with 0.5M HNO3 into cell holder slot 1, close the lid and click OK Wait for the green traffic light. |
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To collect absorbance data click on Start. In the Save As window open the folder corresponding to your section/group. Provide a unique file name. File name should include the date. Then click on Save |
Note: There will be three groups/ instrument. Work together to name the file and load cells |
Note: There will be six groups/ instrument. One instrument will be set to 45°C, the other to 75°C. |
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The Cell Loading Guide will pop-up. Provide names for each cell then load each cell in the proper chamber. When everyone has loaded their cells, close the lid and click on OK. |
Note: The temperature MUST be monitored throughout the run. This should be a shared responsibility. |
Note: The temperature MUST be monitored throughout the run. This should be a shared responsibility. |
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Automatically the SyncStart window opens. Click OK or wait the 2 minutes for the run to start. The run will not start unless the correct temperature has been reached. |
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To view the numerical data click on View and then Report. Save your files to a 31/1 inch floppy disk OR CD OR USB memory stick for further analysis. Be sure to save the files in Spreadsheet Ascii (*.cvs) format |
NOTE: Only analyze your own group's set of data. |
NOTE: Only analyze your own group's set of data. |
To open the files in Excel use the least restrictive criteria to choose the file.
Explore the various icons immediately above the graph for scaling graphs, using a cursor, examining multiple plots, selecting which traces to view and the like.
Please be sure the Cary100, temperature controller and computer are shut down at the end of the day.
Note: lifting up on the knob in the sample compartment will facilitate removal of the cells.
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5.35 / 5.35U Introduction to Experimental Chemistry
Fall 2012
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