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Appendix 1

  • Page ID
    222850
  • Guidelines for Using the Cary 100 UV-Visible Spectrophotometer to Obtain Beer's Law and Kinetics Data

    To launch the software click on the icon Cary WinUV. Follow the column on the left of the table below for common commands and the details for the particular experiment in the appropriate column to the right. Note: items not checked off on the screen or not highlighted for change are not listed below.

      Day 1 Day 2 Day 3
    Experiment Beer's Law Scanning Kinetics Kinetics @550 nm
    Click on the icon: Scan Scanning Kinetics Kinetics

    On the menu bar click on File then on Open Method
    Go to the Look In Window and select Cary \(WinUV^2\) , then

    select:

    5_311_Lambert- Beer_Cobalt Click on Open

    5_311Scanning KineticsCobalt Click on Open
    Note: if it puts up a dialog box about baseline info. close it and go on

    5_311KineticsCobalt Click on Open

    Click on the Setup button. Check that the parameters are correct. See below:

         

    Setup: Cary

    X mode:nanometers Start: 700.00 nm Stop: 300.00 nm
    Y mode:
    Abs

    Y min: 0.00 Ymax: 1.20

    Scan Controls
    Ave time(s)
    0.100 Data interval: 1.000 Scan rate: 600.000

    Temperature Monitor: Block x Show Status

    Display

    X mode:nanometers Start: 700.00 nm Stop: 300.00 nm
    Y mode:
    Abs

    Y min: 0.00 Ymax: 1.00

    Scan Controls
    Ave time(s)
    0.100 Data interval: 1.000 Scan rate: 600.000

    Collect Timing:
    > Simple collect Stage: 1 Cycle(min) : 20.00 Stop(min): 180.00 Temperature Monitor: Block

    x Show Status Display

    Wavelength(nm):

    550.00
    SBW(nm): 1.0 Monitor: Block Ave Time (s): 0.100 Y mode: Abs
    X mode: Min
    Y Min: 0.000
    Y Max: 1.0

    Collect Timing:
    > Simple collect Stage: 1
    Dwell (s): 1.00 Cycle(min) : 20.00 Stop(min): 180.00

    x Show Status Display

    Set-up: Options

    SBW(nm): 2.0

    Source: UV-VIS Source changeover: 350 nm
    > Overlay data

    x Show Status Display

    x Auto Lamp Off SBW(nm): 2.0 Energy: 1.00 Source: UV-VIS Source changeover: 350 nm

    x Show Status Display

    x Auto Lamp Off

    Source: VIS
    Source changeover: 350 nm
    > Individual data
    x Show Status

    Display

    Setup: Baseline

    > Baseline correction

    x Show Status Display

    > Baseline correction

    x Show Status Display

    Not available

    Setup: Accesories 1

    Cells:
    x Use Cell Changer > Select cells
    x check off cells to be used Cell 1-Cell 6
    > 6x6
    Temperature:

    x Automatic

    Temperature

    Setting
    > Temperature

    Controller Block 25.0°C Temperature Display:

    x Block
    x Show Status

    Display

    Cells:
    x Use Cell Changer > Select cells
    x check off cells to be used Cell 1-Cell 6
    > 6x6
    Temperature:

    x Automatic

    Temperature

    Setting
    > Temperature

    Controller Block 60.0°C Temperature Display:

    x Block
    x Show Status

    Display

    Cells:
    x Use Cell Changer > Select cells
    x check off cells to be used Cell 1-Cell 6
    > 6x6
    Temperature:

    x Automatic

    Temperature

    Setting
    > Temperature

    Controller
    Block
    45.0 OR 75.0°C Temperature Display:
    x Block
    x Show Status

    Display

    Setup: Accessories 2

    Not Used Not Used Not Used

    Setup: Accessories 3

    Not Used Not Used Not Used

    Setup: Analyze

    Not Available Not Used Not Used

    Setup: Accessories 3

    Not Available Not Available

    Fill in: 
    Number of Samples
    Sample Names

    Setup: Reports

    Operator:
    Name:
    your names Comment: a brief description

    Options:
    x User Data Form x Graph
    50 %Page Height

    > All traces Peaks
    x Maximum Peak x All Peaks

    XY Pairs Table:
    x Include XY Pairs

    Table
    > Use Actual

    Interval Autoconvert: > Select for

    ASCII(csv) x Show Status

    Display

    Operator:
    Name:
    your names Comment: a brief description

    Options:
    x User Data Form x Graph
    50 %Page Height x Results
    x Parameters

    XY Pairs Table:
    x Include XY Pairs

    Table

    Autoconvert: > Select for

    ASCII(csv) x Show Status

    Display

    Operator:
    Name:
    your names Comment: a brief description

    Options:
    x User Data Form x Graph
    50 %Page Height x Results
    x Parameters

    XY Pairs Table:
    x Include XY Pairs

    Table

    Autoconvert: > Select for

    ASCII(csv) x Show Status

    Display

    Setup: Autostore

    > Storage on (prompt at start)

    x Show Status Display

    > Storage on (prompt at start)

    x Show Status Display

    > Storage on (prompt at start)

    x Show Status Display

    Setup: Click on the OK button. Wait for the green traffic light.

         
     

    To obtain the baseline click on Baseline button
    then:

    Name cell 1 "0.5M HNO3"
    Insert cuvet filled with 0.5M HNO3 into cell holder slot 1, close the lid and click OK

    Wait while the graph of Absorbance vs wavelength appears on the screen. Wait for the green traffic light.

    To obtain the baseline click on Baseline button
    then:

    Name cell 1 "0.5M HNO3"
    Insert cuvet filled with 0.5M HNO3 into cell holder slot 1, close the lid and click OK

    Wait while the graph of Absorbance vs wavelength appears on the screen. Wait for the green traffic light.

    Baseline correction is not used. To zero the instrument at 550 nm click on Zero then: Name cell 1 "0.5M HNO3"

    Insert cuvet filled with 0.5M HNO3 into cell holder slot 1, close the lid and click OK

    Wait for the green traffic light.

    To collect absorbance data click on Start. In the Save As window open the folder corresponding to your section/group. Provide a unique file name. File name should include the date. Then click on Save

     

    Note: There will be three groups/ instrument. Work together to name the file and load cells

    Note: There will be six groups/ instrument. One instrument will be set to 45°C, the other to 75°C.
    Work together to name the file and load cells

    The Cell Loading Guide will pop-up. Provide names for each cell then load each cell in the proper chamber. When everyone has loaded their cells, close the lid and click on OK.

     

    Note: The temperature MUST be monitored throughout the run. This should be a shared responsibility.

    Note: The temperature MUST be monitored throughout the run. This should be a shared responsibility.

    Automatically the SyncStart window opens. Click OK or wait the 2 minutes for the run to start. The run will not start unless the correct temperature has been reached.

         

    To view the numerical data click on View and then Report. Save your files to a 31/1 inch floppy disk OR CD OR USB memory stick for further analysis. Be sure to save the files in Spreadsheet Ascii (*.cvs) format

     

    NOTE: Only analyze your own group's set of data.

    NOTE: Only analyze your own group's set of data.

    To open the files in Excel use the least restrictive criteria to choose the file.

    Explore the various icons immediately above the graph for scaling graphs, using a cursor, examining multiple plots, selecting which traces to view and the like.

    Please be sure the Cary100, temperature controller and computer are shut down at the end of the day.

    Note: lifting up on the knob in the sample compartment will facilitate removal of the cells.

     

    MIT OpenCourseWare
    http://ocw.mit.edu

    5.35 / 5.35U Introduction to Experimental Chemistry
    Fall 2012

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