15.R: Paper Chromatography of Amino Acids (Report)
- Page ID
- 306808
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Part C: Interpretation of Chromatogram
FF value (the distance traveled by the eluting solution): _____________ cm
Record DD values for each eluted spot to the nearest 0.1 cm.
| Amino Acid | Color | Distance(s) Traveled by Eluted Spot(s), D | Rf Value for Eluted Spot |
|---|---|---|---|
| Tyrosine | |||
| Valine | |||
| Glutamine | |||
| Cysteine | |||
| Arginine | |||
| Glutamic acid | |||
| Unknown |
Show calculations for Rf below for the unknown sample:
Identification of unknown #: ____________________
Data Analysis
- This lab employs chromatography to identify amino acids. What other applications can we use chromatography for?
- Are any of the Rf values in the table above the same (or similar, within 0.1)? What would the same or similar Rf values indicate?
- What are the units for Rf values?
- Which amino acid travelled farthest with the mobile phase? What does this indicate about that component’s attraction to the mobile phase? To the stationary phase?
- Which of the amino acids tested if any had an Rf value of zero? What does this indicate about that component’s attraction to the mobile phase? To the stationary phase?
- Why is it necessary to use a pencil to mark the lines and X’s on the paper?