8.R: Osmosis and Dialysis (Report)
- Page ID
- 306780
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Part A: Osmosis
| Distilled H2O | 10% NaCl | 30% NaCl | |
|---|---|---|---|
| Initial weight of dialysis bag (g) | |||
| Final weight of dialysis bag (g) | |||
| Change in weight (g) |
B. Identification Tests for Chloride, Glucose and Starch
| Test Performed | Reagent Added | Observation with test solution | Observation with water (control) |
|---|---|---|---|
| Chloride | |||
| Glucose | |||
| Starch |
C. Dialysis
| OBSERVATIONS FOR: | |||
|---|---|---|---|
| Time | Chloride test | Glucose test | Starch test |
| 0 minutes | |||
| 15 minutes | |||
| 30 minutes | |||
| Content of bag | |||
Data Analysis/ Post-lab Questions
- Imagine that the dialysis bag you used in part A is a ‘cell’ and that the solution inside the bag is intracellular fluid.
- Which solution is isotonic to the fluid inside the bag?
- Which solution is hypertonic to the fluid inside the bag?
- Which solution is hypotonic to the fluid inside the bag?
- What would have happened in each of the three beakers in part A if the bags were filled with distilled water instead of the 30% sodium chloride solution?
- In part C which substances were found in the solution outside the dialysis bag?
- How did these substances in question 3 go into the solution outside the bag?
- What substances were retained inside the bag in part C? Why were they retained?
- 0.9 % (m/v) NaCl and 5% (m/v) glucose solutions are said to be isotonic with respect to the intracellular fluid of red blood cells. A red blood cell placed in these solutions would undergo neither crenation nor hemolysis (i.e. no change in the red blood cell would occur). Given this information predict if a red blood cell would undergo crenation, hemolysis, or no change in the following solutions:
- 2% (m/v) glucose
- 2% (m/v) NaCl
- 0.1% (m/v) NaCl
- 10% (m/v) glucose
- Are the solutions given above isotonic, hypotonic or hypertonic with respect to the intracellular fluid of a red blood cell?