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Procedures: The Module

  • Page ID
    63691
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    1. Outline the experiments you must do to determine the retention factor (capacity factor) for 4 NSAIDs.
    2. Determine the retention factor (capacity factor) for flurbiprofen, naproxen, sulindac, and tolmetin. These values should be reported as mean values determined from triplicate runs (n = 3). For these runs, the background BGE is 25 mM CAPs, 100 mM SDS buffered to pH 10. You must use a ~25 micron inner diameter fused silica capillary ~42 cm in total length, ~32 cm to the window, 20,000 V.

      Free Zone Data
      Lw = ______cm, Lt = ______cm, Vsep = ______V

      Migration Time μ eof (CE)

      Marker

      Trial

      1

      2

      3

      Trial

      1

      2

      3

      DMF
      Migration Time μ app

      Analyte

      Trial

      1

      2

      3

      Trial

      1

      2

      3

      Tolmetin

      Naproxen

      Flurbiprofen

      Sulindac
      μ eph

      Analyte

      Trial

      1

      2

      3

      AVE

      Tolmetin

      Naproxen

      Flurbiprofen

      Sulindac

      MEKC DATA
      Lw = ______cm, Lt = ______cm, Vsep = ______V
      Migration Time μ eof (MEKC)

      Marker

      Trial

      1

      2

      3

      Trial

      1

      2

      3

      DMF

      n-dec
      Migration Time k'

      Analyte

      Trial

      1

      2

      3

      Trial

      1

      2

      3

      AVE

      Tolmetin

      Naproxen

      Flurbiprofen

      Sulindac
    3. Lab Practical

      Through a faculty mentor or collaborator, you should arrange to receive an unknown solution containing some combination of flurbiprofen, naproxen, sulindac, and tolmetin diluted in BGE at a concentration above the limit of quantification for your CE system. Once you receive the unknown solution, you are to determine the analyte composition and concentration in the solution. Your final report should include pertinent data and a clear explanation of your results. When you report your final value, be sure to include uncertainty. You should have access to standard solutions as necessary.


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