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Chemistry LibreTexts

Procedures: The Module

  1. Outline the experiments you must do to determine the retention factor (capacity factor) for 4 NSAIDs.
  2. Determine the retention factor (capacity factor) for flurbiprofen, naproxen, sulindac, and tolmetin. These values should be reported as mean values determined from triplicate runs (n = 3). For these runs, the background BGE is 25 mM CAPs, 100 mM SDS buffered to pH 10. You must use a ~25 micron inner diameter fused silica capillary ~42 cm in total length, ~32 cm to the window, 20,000 V.

    Free Zone Data
    Lw = ______cm, Lt = ______cm, Vsep = ______V

      Migration Time μ eof (CE)

     

    Marker

    Trial

    1

     

    2

     

    3

    Trial

    1

     

    2

     

    3

    DMF            
      Migration Time μ app

     

    Analyte

    Trial

    1

     

    2

     

    3

    Trial

    1

     

    2

     

    3

    Tolmetin

               

    Naproxen

               

    Flurbiprofen

               
    Sulindac            
      μ eph          

     

    Analyte

    Trial

    1

     

    2

     

    3

     

    AVE

       

    Tolmetin

               

    Naproxen

               

    Flurbiprofen

               
    Sulindac            

     

    MEKC DATA
    Lw = ______cm, Lt = ______cm, Vsep = ______V
      Migration Time μ eof (MEKC)  

     

    Marker

    Trial

    1

     

    2

     

    3

    Trial

    1

     

    2

     

    3

     

    DMF

                 
    n-dec              
      Migration Time k'  

     

    Analyte

    Trial

    1

     

    2

     

    3

    Trial

    1

     

    2

     

    3

     

    AVE

    Tolmetin

                 

    Naproxen

                 

    Flurbiprofen

                 
    Sulindac              
  3. Lab Practical

    Through a faculty mentor or collaborator, you should arrange to receive an unknown solution containing some combination of flurbiprofen, naproxen, sulindac, and tolmetin diluted in BGE at a concentration above the limit of quantification for your CE system. Once you receive the unknown solution, you are to determine the analyte composition and concentration in the solution. Your final report should include pertinent data and a clear explanation of your results. When you report your final value, be sure to include uncertainty. You should have access to standard solutions as necessary.