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Chemistry LibreTexts

Molecular Luminescence Spectroscopy

General Reference: http://www.prenhall.com/settle/chapters/ch26.pdf

  1. Know the possible deactivation processes available to a molecule which absorbs a photon and undergoes an electronic energy transition.

    http://teaching.shu.ac.uk/hwb/chemistry/tutorials/molspec/lumin1.htm

    http://teaching.shu.ac.uk/hwb/chemistry/tutorials/molspec/lumin3.htm

  2. Be able to explain the impact of molecular structure, temperature, solvent, and concentration on fluorescence.

    Harvey 10.6.3

    http://www.springerlink.com/content/qp463n1w010033h7/fulltext.pdf -- solvent effects

  3. Be able to explain the various instrumental components and diagrams for fluorimeters and spectrofluorimeters.

    Harvey 10.6.1

    http://www.fluorescence-foundation.org/lectures/genova2006/lecture3.ppt (This site asks for user name and password, but opens without them when “cancel” is selected)

    http://www.instrumentalchemistry.com/liquidphase/pages/fluoresence.htm

  4. Be able to acquire and process data from an analysis. This includes, but is not limited to (quantitative analysis): obtaining a signal for a blank, standards, and unknown; taking into account how the sample was processed (sample blank), which includes dilutions of both the sample and standards; plotting the data (Signal vs. Concentration) and obtaining a calibration curve; calculating a limit of detection for the analyte.

    Harvey 10, end of chapter problems. 40-43