Inquiry-based in class Questions: (Designed to be presented to the students preceding the textual materials).
(1) What is an obvious physical difference between an immunoglobulin (antibody), which is a protein, and glucose, which is a monosaccharide?
(2) When would it be useful to separate immunoglobulins from glucose in blood?
(3) How could you devise an experiment to separate immunoglobulins from glucose utilizing the physical difference between the molecules determined in (1)?
(4) Depicted below are three different sized molecules. Next to them is shown a pore. What would happen as each molecule interacted with the pore? How would this effect the retention time of each molecule?
(5) Other chromatographic methods depend on specific interactions between the molecules being separated and the surface of the solid support. What effect might such specific interactions have on the method you came up with to separate glucose from immunoglobulins? Can you think of how you might limit specific interactions between the molecules and the surface?
(6) Would you expect glucose or immunoglobulins to produce a broader peak profile when dissolved in buffer solution at a similar concentration? Which of the peaks below is most likely to belong to glucose? Which peak is most likely to belong to an immunoglobulin? Explain.
(7) Would it be easier to separate glucose from an immunoglobulin or two immunoglobulins from each other? Explain.